MRPrimerW

Search valid primers for multiple targets of qPCR experiments without matching off-targets



Basic Parameters
Species Search by

For text

If "Search by" is Keyword, use comma(,) as separator for multiple targets



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TaqMan probe design


Advanced Settings

Single filtering

Primer melting temperatures (Tm)  
GC content (%)  
Primer length  
Self-complementarity
3' end Self-complementarity
Contiguous residue
End stability
Hairpin

Pair filtering

PCR Amplicon size  
Length difference
Temperature difference
Pair-complementarity
3' end Pair-complementarity